DNA

Part:BBa_K5507003:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


sacB-5‘


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sacB gene was selected as a homologous recombination site because knockout of this gene had no metabolic effects under a glucose condition, under which all the gene manipulation experiments were conducted in this condition. We will replace the location of the sacB gene with the overexpression of pgi.


Source

sacB-5 is derived from the K. xylinus genome.

References